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. Author manuscript; available in PMC: 2022 Nov 15.
Published in final edited form as: Arch Biochem Biophys. 2021 Oct 2;712:109051. doi: 10.1016/j.abb.2021.109051

Table 1.

Components required for the formation of nanodiscs.

Sample DMPC (mg) 200 mM detergent (μL) 1.4 mM MSP (μL) 10× buffer (μL) H2O (μL) Total Vol. (μL) MSP: DMPC* Detergent: DMPC*
Cholate 2.0 30 13 30 227 300 1:160 2:1
CHAPS 2.0 30 13 30 227 300 1:160 2:1
OG 2.0 60 13 30 197 300 1:160 4:1
*

Molar ratios are based on those presented in Ritchie et al [11].