Figure 2.

Re-expression of wild-type (WT; α5^−/−-Lv-α5WT) and polymorphic (α5^−/−-Lv-α5SNP) α5 in the ventral tegmental area (VTA) of α5^−/− mice. (a) Scheme of lentiviral vectors, see Supplementary Information for details. (b) Localization of lentivirus reporter gene eGFP expression in VTA. Arrowheads indicate tyrosine hydroxylase (red) and eGFP (green) co-expression by DA cells. (c) Example of a recorded neuron (single plane): tyrosine hydroxylase, eGFP and biocytine identify, respectively, DA cells (red), the neuron re-expressing the α5 subunit (green), and a recorded cell (blue). eGFP, enhanced green fluorescent protein.