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. 2021 Oct 1;60(44):23835–23841. doi: 10.1002/anie.202110327

Figure 2.

Figure 2

Characterization of StrepEncSM loaded with eGFP. Four cargo protein constructs, tagged with one short peptide of the putative ELS (6 aa, 9 aa, 12 aa and 19aa long; ctrl. 0 aa and no eGFP). A) Native PAGE analysis of the co‐purified constructs and analysis by Coomassie staining and in‐gel fluorescence. B) Quantitative assessment of the fluorescence intensities detected by in‐gel fluorescence using ImageJ. The analysis was done in triplicate. Error bars represent ± SEM. Key: amino acid (aa), encapsulin localization sequence (ELS).