Figure 5.

Uncaging reaction of 1 (125 μM, 1 equiv) to 2. Anaerobic labeling and washing steps with standard aerobic reaction conditions. Yields were determined by fluorescence intensity in three independent experiments (λ _ex=339 nm, λ _em=439 nm). Error bars represent ± SEM. Key: ^StrepEnc_SM{HaloTag‐PEG₃‐Ru} (black square), HaloTag‐PEG₃‐Ru (blue triangle), ^StrepEnc_SM{GFP} (green triangle), CA‐PEG₃‐Ru (5) (turquoise diamond).