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. 2021 Oct 1;60(44):23835–23841. doi: 10.1002/anie.202110327

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© 2021 The Authors. Angewandte Chemie International Edition published by Wiley-VCH GmbH

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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Fluorescence microscopy and bright‐field images of J774A.1 monocytes treated with ^StrepEnc_SM{HaloTag‐PEG₃‐Ru} + 1, CA‐PEG₃‐Ru (5) + 1 and 1 only (negative controls). Cells were seeded and treated with 1 μM ^StrepEnc_SM{HaloTag‐PEG₃‐Ru} in DMEM overnight. Subsequently, 1 (20 μM) was applied to the cell supernatant. Following incubation and washing, images were acquired with a digital inverted microscope. Fluorescence microscopy images of monocytes treated with encapsulin show fluorescence from uncaging product 2 that is localized within distinct vesicles. Fluorescence microscopy images show no blue fluorescence in cells treated with 1 only. Key: Dulbecco's Modified Eagle Medium (DMEM).