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Sample Exo9 reconstitution
| Component | Molecular weight (Da) | mg/mL | μM | Multiplier | nmol needed | mL needed |
|---|---|---|---|---|---|---|
| Smt3-Rrp41/Rrp45 | 74,000 | 17 | 230 | 1.0 | 42 | 0.19 |
| Smt3-Rrp42/Mtr3 | 81,000 | 10 | 140 | 1.0 | 42 | 0.29 |
| Smt3-Rrp46/Rrp43 | 80,000 | 7.6 | 95 | 3.0 | 130 | 1.4 |
| Smt3-Csl4 | 44,000 | 26 | 590 | 3.0 | 130 | 0.22 |
| Smt3-Rrp4 | 51,000 | 11 | 220 | 1.0 | 42 | 0.2 |
| Smt3-Rrp40 | 39,000 | 14 | 360 | 1.8 | 74 | 0.21 |
| Ulp | 26,000 | 2.0 | 77 | 0.010 | 0.4 | 0.005 |
| Buffer R1 | 7.5 |
