Table 4:

Substrate mixes for Exo14 biochemical reconstitutions

Component	Stock concentration (mM)	ds17A10, AMP-PNP	ds17A10, ATP	ss17A10, AMP-PNP	ss17A10, ATP
AMP-PNP	50	4.4 μL	0 μL	4.4 μL	0 μL
ATP	50	0 μL	4.4 μL	0 μL	4.4 μL
ds17A10 RNA	0.001	2.2 μL	2.2 μL	0 μL	0 μL
ss17A10 RNA	0.001	0 μL	0 μL	2.2 μL	2.2 μL
RNase inhibitor	40 U/μL	2.5 μL	2.5 μL	2.5 μL	2.5 μL
RNA decay buffer		191 μL	191 μL	191 μL	191 μL
Total		200 μL	200 μL	200 μL	200 μL