FIG. 1.

(A) Quantitative analysis of the interaction between Phd, PhLOP1, truncated PhLOP1, and bCRX. Either AD-bCRX or the AD vector was cotransformed with the indicated BD fusion constructs into the yeast reporter strain Y190. The transformants were processed for the β-Gal activity assay. Data are means ± standard errors of two independent experiments done in triplicate. The β-Gal activity is expressed in standard units (1,000 × OD₄₂₀/time [minutes] × OD₆₀₀). (B) Schematic alignment of the amino acid sequences of the Phd isoforms. Open bars, identical sequences among different isoforms; solid bars, Gβγ BD (TGPKGVINDWR) (64). Domains unique to either PhLP1 or PhLOP2 are shown by bars with different patterns. Arrows identify the Gβγ BD, the phosphorylation site (Ser73), and the CRX BD.