Skip to main content
. Author manuscript; available in PMC: 2021 Nov 17.
Published in final edited form as: Nat Microbiol. 2021 Apr 19;6(6):731–745. doi: 10.1038/s41564-021-00890-3

Extended Data Fig. 7. Flow cytometry gating (part 2).

Extended Data Fig. 7

A: Flow cytometry gating scheme utilized to measure membrane damage in PECs after treatment with PBS control (top) and leukocidins (LukED is shown as an example, bottom) using Fixable Viability Dye eFluor™ 450 (Figure 6A).

B: Flow cytometry gating scheme utilized to measure membrane damage in Lenti-X 293T cells expressing C-terminal GFP-tagged wildtype HVCN1 and chimeric proteins (human HVCN1 is shown as an example) following treatment with PBS control (top) and CC30 LukAB (bottom) using Fixable Viability Dye eFluor™ 450 (Figure 6D).