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. Author manuscript; available in PMC: 2021 Nov 17.
Published in final edited form as: Nat Microbiol. 2021 Apr 19;6(6):731–745. doi: 10.1038/s41564-021-00890-3

Figure 2. CC30 and CC45 LukAB kill cells lacking CD11b/CD18.

Figure 2.

A: Flow cytometric analysis of surface CD11b levels of scramble shRNA and ITGAM shRNA (CD11b-depleted) expressing THP1 cells. Histograms are representative of two independent experiments. B-C: Intoxication of scramble shRNA (B) and ITGAM shRNA (C) expressing THP1 cells with indicated concentration of different LukAB variants. Data from three independent experiments are shown as mean values ±SD. D: Intoxication of scramble shRNA, ITGAM shRNA, and ITGB2 (CD18) shRNA expressing PMN-like HL60 cells with CC8, CC30, and CC45 LukAB (2.5 μg/ml). Data from three independent experiments are represented as mean values ±SD. B-D: Cell viability was measured with Cell Titer and statistical significance was determined by one-way ANOVA, numbers indicate P values. E: Binding of biotinylated CC8 and CC30 LukAB to the CD11b I-domain measured by ELISA. Data from three independent experiments are represented as mean values ±SD. Statistical significance was determined by two-way ANOVA (****, P ≤ 0.0001; ***, P ≤ 0.001; **, P ≤ 0.01; ns, not significant). Also refer to Extended Data Figures 3 and 6.