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eFx‐mediated aminoacylation of microhelix RNA (FAM‐MiHx_23 b). Reaction mixtures were analyzed on a 20 % polyacrylamide gel by detection of the FAM label. Acylation efficiencies were calculated by densitometric analysis of RNA bands. Substrates tested: ClAc−W−CME, ClAc−4CNW−CME 7, ClAc−AzAla−CME 8.
