FIGURE 1.

Topical application of amygdalin analogue cream ameliorates psoriasis‐like severity in DKO* mice. (A) Experimental timeline to induce psoriasis‐like disease in 8‐week‐old mice with four consecutive doses of tamoxifen injections (2 mg/dose). Six days later after psoriasis‐like induction, mice were visually evaluated and only mice with an initial psoriasis‐like severity score = 2 were used. One hundred microlitre of amygdalin analogue and vehicle creams was blindly applied in both ears every day during 14 days in randomly chosen DKO* mice. Wild‐type (WT) and DKO* mice without cream were used as control groups. n > 7 per group. (B) DKO* mice classified according to the psoriasis‐like severity score (1 minimum–5 maximum) after treatment with amygdalin analogue or vehicle cream at day 20 after disease induction. DKO* without cream as control without treatment. n = 7 for DKO* mice and n = 9 for each vehicle and amygdalin‐treated mice. Statistical significance ***p < 0.0001. p value was determined by χ ² statistics. (C) Representative images of haematoxylin and eosin (H&E) staining of ear sections from DKO* without treatment, DKO* treated with amygdalin cream and DKO* mice treated with vehicle cream at day 20 after psoriasis‐like induction. (D, E) Ear and epidermis thickness measurement in control (WT), DKO* before treatment at day 6, DKO* without treatment at day 20 and DKO* mice treated with amygdalin analogue and vehicle creams at day 20. n > 5 per group. Statistical significance *p < 0.05 (t‐student two‐tailed test relative to controls and vehicle group)