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. 2021 Nov 17;21:607. doi: 10.1186/s12935-021-02306-y

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — MF inhibits functionality, growth capacity, and viability of UM cell lines in a concentration-related manner. Graphs represent the level of cellular functionality or viability, respectively as detected via a CCK8 colorimetric assay (A) or Trypan Blue exclusion assay (B) after cells were treated with increasing concentrations of MF (0, 5, 10, 20, or 40 μM) for 72 h. C Growth curves obtained through Incucyte live cell imaging system, tracking cellular confluency. In A and B, data were analyzed using two-way ANOVA followed by Dunnett’s multiple comparison test. In C, data were analyzed using repeated measures ANOVA followed by Tuckey’s multiple comparison test.* Indicates p < 0.05, ** indicates p < 0.01, whereas *** indicates p < 0.001 compared against vehicle-treated controls