Fig. 2 .

Long-term toxicity of MF towards UM cell lines and the consequence of MF withdrawal. UM cells were treated with MF at concentrations of either 0, 20, or 30 μM for 72 h and imaged every 6 h in the Incucyte. Following the initial 72 h, media was aspirated, replaced with regular growth media, and placed back into the Incucyte to be imaged for another 72 h. The red arrows at 72 h indicate the moment in which MF was removed from the media. Data were analyzed using repeated measures ANOVA followed by Tuckey’s multiple comparison test. * Indicates p < 0.05, ** indicates p < 0.01, whereas *** indicates p < 0.001 compared against vehicle-treated controls