Fig. 3 .

MF induces accumulation of hypodiploid DNA content and DNA ladder. A Quantification of particles with hypodiploid DNA content upon 72 h of MF treatment in a panel of UM cell lines. The hypodiploid DNA content corresponds to the Sub-G1 DNA content extrapolated when performing the cell cycle analysis of the cells treated with MF (the quantitative details are shown in the green-stained sections of the histograms in Additional file 3: Fig. S3). B A similar experiment was done in which all floating and adherent cells were pelleted, gDNA isolated, subjected to agarose electrophoresis, stained with SYBR Gold nuclei acid stain, and imaged. A 100 base pair (bp) maker was run in parallel. −: vehicle; + : 40 µM MF