Figure 3.

TTField-mediated inhibition of migration is stronger upon combination with hyperthermia. AsPC-1, BxPC-3 and Bx-GEM cells were seeded at a density of 4,000 cells/well in 96-well plates and grown to 90% confluency overnight. The cell layer was scratched with the tip of a 100-µL pipet, followed by treatment of the cells with TTFields at 38.5°C for 24 h. The controls (CO) were incubated at 38.5°C without exposure to TTFields. The closure of the wounded region was evaluated by microscopy at 0, 12 and 24 h after scratching. The gap width was measured using ImageJ. Representative images are shown on the left, and the means are shown in bar graphs on the right. The data are presented as the means ± SD. *P < 0.05, **P < 0.01.