FIG. 6.
Recombinant TBP stimulates transcription from the core domain in an extract defective for UAF. Extract from strain RLY07 was used to transcribe templates either mutated in the upstream domain (LSup, lanes 1 through 4), mutated in the core domain (LScore, lanes 5 and 6), or having the upstream domain deleted (Δ−42, lanes 7 through 11). Template concentrations were 10 μg/ml, and recombinant TBP (rTBP) was added to the reaction mixtures as indicated. PhosphorImager quantitation indicates about a threefold stimulation of transcription between lanes 1 and 3 and about a fourfold stimulation between lanes 7 and 10.