TABLE 1.
Yeast strains and plasmids
| Strain or plasmid | Description |
|---|---|
| Strains | |
| RLY01 | MATα ade2-1 ade3::hisG ura3-1 his3-11 trp1-1 leu2-112 can1 |
| RLY02 | MATα ade2-1 ade3::hisG ura3-1 his3-11 trp1-1 leu2-112 can1 Δrrn11::HIS3 pNOY103 |
| RLY05 | MATα ade2-1 ade3::hisG ura3-1 his3-11 trp1-1 leu2-112 can1 Δrrn7::HIS3 pNOY103 |
| RLY06 | MATα ade2-1 ade3::hisG ura3-1 his3-11 trp1-1 leu2-112 can1 Δrrn6::HIS3 pNOY103 |
| RLY07 | MATα ade2-1 ade3::hisG ura3-1 his3-11 trp1-1 leu2-112 can1 Δrrn5::HIS3 pNOY103 |
| RLY08 | MATα ade2-1 ade3::hisG ura3-1 his3-11 trp1-1 leu2-112 can1 Δrrn9::TRP1 pNOY103 |
| RLY09 | MATα ade2-1 ade3::hisG ura3-1 his3-11 trp1-1 leu2-112 can1 Δrrn10::HIS3 pNOY103 |
| RLY4811 | Isogenic to RLY07, which carries the temperature-sensitive allele of the RPA190 gene |
| Plasmids | |
| pNOY103 | 2μm-, URA3-, and ADE3-expressing GAL7 35S rRNA (19) |
| pSII223 | YEp351 expressing TBP under the control of its own promoter (S. Hahn, Fred Hutchinson Cancer Research Center) |
| pRS315 | ARS CEN LEU2 vector (19a) |
| pRS315RRN5 | pRS315 expressing RRN5 under the control of its own promoter |