FIG 2.

Time course reaction of CRISPR-Cas12a-mediated detection of SARS-CoV-2. The fluorescence was measured at different time points using 10¹⁰ copies/μl SARS-CoV-2 nucleoprotein (NP) plasmid DNA as the template and different NP-specific guide RNAs crRNA-N1 (A), crRNA-N2 (B), crRNA-N3 (C), crRNA-N4 (D), and crRNA-N5 (E), respectively, or using 10¹⁰ copies/μl wild-type and mutant spike (S) gene as the template and guide RNA crRNA-S (F). In each panel, assay specificity was validated with nucleic acid samples of common human coronavirus (HCoV) 229E, HCoV OC43, and HCoV HKU1, rhinovirus (HRV), adenovirus (ADV), respiratory syncytial virus (RSV) A and B, human bocavirus (HBoV), human metapneumovirus (HMPV), human parainfluenza virus (HPIV-1 and HPIV-4), and Mycoplasma pneumoniae, as well as HIV-1, HBV, HCV, Chlamydia trachomatis, and Treponema pallidum. The mean value of fluorescence of three independent experiments is presented. No input indicates negative control of no-plasmid DNA.