FIG 3.

Low limit of detection (LOD) for CRISPR-Cas12a direct detection (left panels) and the integrated PCR/CRISPR-Cas12a detection (right panels). (A to H) Fluorescence was measured at different time points using different amount of SARS-CoV-2 plasmid DNA of nucleoprotein (A and B), ORF8 (C and D), and wild type (E and F) or mutant (G and H) spike gene as the template. No input indicates negative control of no-plasmid DNA. The mean value of fluorescence of three independent experiments is presented.