FIG 5.

Detection of the single nucleotide mutations across SARS-CoV-2 spike protein by PCR-CRISPR/Cas12a-mediated assay. Different concentrations of SARS-CoV-2 DNA were used as the template and amplified by PCR followed by detection of CRISPR-Cas12a-mediated assay. Wild type (WT) and mutant (Mut) template are labeled in red and green, respectively. The position and name of the amino acid for the templates are indicated in the brackets. The name of the crRNA and the corresponding mutant amino acid were put at the top of each panel. The fluorescence values represent the mean ± standard deviation (SD) of 3 replicates. Two-tailed student's t test was used to analyze the difference between wild-type and mutant template. n.s., not significant; *, P < 0.05; **, P < 0.01; ****, P < 0.0001; WT, wild type; Mut, mutant; no input, negative control with no plasmid DNA.