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. 2021 Nov 17;9(3):e01017-21. doi: 10.1128/Spectrum.01017-21

FIG 9.

FIG 9

Heatmap of detection results for SARS-CoV-2. (A) Four virus isolates, including SARS-CoV-2 wild-type strain and alpha, beta, and delta variants were evaluated in CRISPR-Cas12a system using mutation specific crRNAs, i.e., cr-RNA-S-417K, crRNA-S-417N, crRNA-S-452R, crRNA-S-478T, crRNA-S-478K, and crRNA-S-484K, crRNA-S-484Q, crRNA-S-501N, and crRNA-S-614D. The corresponding signature residues detected by the specific crRNAs are indicated in the boxes of the heatmap. (B) Thirty-two clinical samples in a panel of 5 wild-type strains, 11 alpha variants, 8 beta variants, and 8 delta variants were evaluated in CRISPR-Cas12a system using mutation-specific crRNAs. The scale bar shows the range of fluorescence values represented by color change from blue to red.