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. Author manuscript; available in PMC: 2022 Jan 1.
Published in final edited form as: Methods Mol Biol. 2021;2218:219–244. doi: 10.1007/978-1-0716-0970-5_18

Figure 1.

Figure 1.

A generalized diagram and approximate timeline of Methods 3.1 – 3.3, representing the overall workflow required for two-color fluorescence in situ hybridization with microtubule labeling. Note that embryo fixation and RNA probe preparation (Methods 3.1 and 3.2) can be performed well in advance of Method 3.3 (or other downstream uses, such as Method 3.4).