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. Author manuscript; available in PMC: 2022 Jan 1.
Published in final edited form as: Methods Mol Biol. 2021;2218:219–244. doi: 10.1007/978-1-0716-0970-5_18

Figure 2.

Figure 2.

Generalized schematic of PCR based RNA probe synthesis. This diagram depicts an RNA probe designed to target a region spanning an exon-exon junction, but other design strategies, such as including portions of the 5’ or 3’ UTR, can be employed (see Note 1).