Figure 3.

Micrograph of a germ plasm aggregate (nanos) and microtubules surrounding a nucleus in a fixed 2-hour post-fertilization zebrafish embryo. Using the combined in situ/immunofluorescence protocol described in Method 3.3, digoxigenin-labeled probes targeting nanos RNA and antibodies targeting alpha-tubulin were used to simultaneously visualize an RNA–rich structure (germ plasm) with microtubules and a DAPI-stained nucleus. Image is a 2D projection of a Z-stack acquired on a Zeiss LSM510 confocal microscope.