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. Author manuscript; available in PMC: 2022 Jan 1.
Published in final edited form as: Methods Mol Biol. 2021;2218:219–244. doi: 10.1007/978-1-0716-0970-5_18

Figure 4.

Figure 4.

Micrograph of F-actin arcs and germ plasm RNPs (nanos) in the blastodisc periphery of a fixed 2-cell (45 minutes post-fertilization) zebrafish embryo. As described in Method 3.4, fluorophore-conjugated phalloidin was incorporated with the fixation step to label F-actin (pseudocolored magenta here) followed by in situ hybridization to visualize fluorescein-labeled probes bound to nanos RNA. Image is a 2D projection of a Z-stack acquired on a Zeiss LSM780 confocal microscope.