| PCR amplification mix (per reaction) | |
|---|---|
| Amount (μl) | |
| Nuclease free H2O | 3.85 |
| dNTP/buffer mix | 17.9 |
| 10mM Fwd primer + 10mM Rev primer mix | 0.15 |
| cDNA (~10-100ng) | 3.00 |
| EconoTaq DNA polymerase | 0.10 |
| Total reaction volume = | 25.0 |
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