| Step | Temperature | Time |
|---|---|---|
| Initial denaturation | 95 °C | 2 min |
| 45 Cycles | 95 °C 45-65 °C (see note 11) 72 °C |
30 sec 15-60 sec 1 min/kb |
| Final extension | 72 °C | 5 min |
| Hold | 4 °C | - |
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