| RNA probe synthesis mix (per reaction) | |
|---|---|
| Amount (μl) | |
| Nuclease-free H2O | 12.0 |
| 10X transcription buffer | 2.00 |
| RNA labeling mix (conjugated UTPs) | 2.00 |
| Template DNA (~1-2 μg from PCR or plasmid) | 1.00 |
| Ribolock RNase inhibitor | 1.00 |
| T7 RNA polymerase | 2.00 |
| Total reaction volume = | 20.0 |
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