| Cytoskeleton fixative/Phalloidin-labeling mix (per tube of embryos) |
||
|---|---|---|
| Amount (μl) | Final concentration | |
| 4% paraformaldehyde (PFA) | 965.5 | - |
| 25% glutaraldehyde | 10.0 | 0.25% |
| 500 mM EGTA | 10.0 | 5 mM EGTA |
| Triton X-100 | 2.0 | 0.2% |
| 1:200 conjugated phalloidin | 12.5 | 0.2 units/ml |
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