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. Author manuscript; available in PMC: 2022 Jan 1.
Published in final edited form as: Methods Mol Biol. 2021;2218:219–244. doi: 10.1007/978-1-0716-0970-5_18
dNTP/buffer mix (aliquots stored at −20C)
Amount (mL) Final concentration
1 M MgCl2 0.393 2.05 mM
1 M pH 8.4 Tris-HCl 2.613 13.7 mM
1 M KCl 13.092 68.3 mM
BSA (100 mg/ml) 3.468 27.1 mM
100 mM dATP 0.262 137 μM
100 mM dCTP 0.262 137 μM
100 mM dGTP 0.262 137 μM
100 mM dTTP 0.262 137 μM
Nuclease-free H2O 171.12 -