FIG 1.

EDTA and/or taurolidine effects on a PDC-associated biofilm: microbial growth during treatment and biofilm production at time 48 h. PDC pieces (0.5 cm) were contaminated with BLI-Pseudomonas (10⁵/ml) for 24 h. Then, the PDC-associated biofilm (24 h-old) was exposed to EDTA and/or taurolidine at the indicated doses from 24 h to 48 h, at 37°C. During such incubation time, the BLI signal was recorded (A) and, at the end of the treatment, the PDC were washed again and the persistent 48-h-old biofilm was assessed (B). The values were expressed as the mean ± SEM of the RLU of 8 replicates obtained in two independent experiments. Statistical analysis was performed according to Student’s t test using GraphPad Prism 8. **, P < 0.005; ***, P < 0.001.