. 2021 Nov 17;9(3):e01047-21. doi: 10.1128/Spectrum.01047-21

TABLE 2.

Secretory profile of BLI-Pseudomonas undergoing two treatments with EDTA/taurolidine (assessment of the four autoinducers [AI] by HPLC-MS)^a^,^b^,^c

	3-oxo-C12-HSL		C4-HSL		PQS		IQS
Treatment	Peak area	Fold change treated vs untreated	Peak area	Fold change treated vs untreated	Peak area	Fold change treated vs untreated	Peak area	Fold change treated vs untreated
24-h-old biofilm
TSB	1.14E-06		2.73E-07		1.63E-09		7.10E-03
1st treatment
SB	1.04E-06	1	2.54E-07	1	1.91E-09	1	2.97E-06	1
EDTA 2.5%	8.02E-05	0.77	5.60E-06	0.22	2.40E-05	0.001	ND
Tauro 0.5%	3.45E-05	0.33	3.20E-06	0.13	3.67E-07	0.19	ND
EDTA 2.5% + Tauro 0.5%	ND		2.50E-06	0.10	1.54E-07	0.08	ND
Regrowth
SB	2.17E-06	1	2.90E-07	1	4.68E-09	1	5.08E-06	1
EDTA 2.5%	2.43E-06	1.1	2.85E-07	1.00	3.97E-09	0.85	4.11E-06	0.82
Tauro 0.5%	2.75E-06	1.3	2.55E-07	0.89	4.09E-09	0.87	3.49E-06	0.70
EDTA 2.5% + Tauro 0.5%	2.67E-06	1.2	2.34E-07	0.82	3.93E-09	0.84	3.66E-06	0.73
2nd treatment
SB	1.32E-06	1	2.07E-07	1	8.90E-08	1	4.82E-06	1
EDTA 2.5%	1.04E-06	0.79	2.36E-07	1.1	1.04E-09	1.17	5.22E-06	0.3
Tauro 0.5%	1.27E-06	0.96	1.47E-06	0.1	ND		ND
EDTA 2.5% + Tauro 0.5%	3.42E-05	0.26	1.25E-06	0.1	4.95E-07	0.06	ND

Supernatants from PDC pieces, contaminated and treated or not treated with EDTA and/or taurolidine, were tested by HPLC-MS. The indicated AI were investigated.

By chromatogram analysis, the different elution peaks were identified, and their areas were used for semiquantitative evaluation of each specific product. The fold change was calculated with respect to the corresponding SB control. The results shown are from a pool of three replicates of a representative experiment out of two performed.

ND, not detectable.