TABLE 4.
Secretory profile of BLI-Pseudomonas undergoing two treatments with EDTA/taurolidine (assessment of four different pyoverdines by HPLC-MS)a,b,c
| Treatment | Succ-p-Ser-Y |
Succa-P-Ser-Y |
PyE |
PyD |
||||
|---|---|---|---|---|---|---|---|---|
| Peak area | Fold change treated vs untreated | Peak area | Fold change treated vs untreated | Peak area | Fold change treated vs untreated | Peak area | Fold change treated vs untreated | |
| 24-h-old biofilm | ||||||||
| TSB | 1.96E- 04 | 5.20E-04 | 1.00E-06 | 2.70E-05 | ||||
| 1st treatment | ||||||||
| SB | 1.22E-04 | 1 | 5.90E-04 | 1 | 1.05E-06 | 1 | 5.08E-05 | 1 |
| EDTA 2.5% | ND | ND | ND | 7.40E-04 | 0.15 | |||
| Tauro 0.5% | ND | ND | ND | ND | ||||
| EDTA 2.5% + Tauro 0.5% | ND | ND | ND | ND | ||||
| Regrowth | ||||||||
| SB | 2.12E-04 | 1 | 7.77E-04 | 1 | 3.08E-06 | 1 | 1.46E-06 | 1 |
| EDTA 2.5% | 1.97E-04 | 0.9 | ND | ND | 7.90E-05 | 0.54 | ||
| Tauro 0.5% | ND | ND | 6.70E-05 | 0,22 | 8.14E-05 | 0.56 | ||
| EDTA 2.5% + Tauro 0.5% | ND | ND | ND | 6.60E-05 | 0.45 | |||
| 2nd treatment | ||||||||
| SB | 9.30E-03 | 1 | 1.07E-04 | 1 | 1.61E-05 | 1 | 7.16E-05 | 1 |
| EDTA 2.5% | ND | ND | ND | ND | ND | |||
| Tauro 0.5% | ND | ND | ND | ND | ND | |||
| EDTA 2.5% + Tauro 0.5% | ND | ND | ND | ND | ND | |||
a
Supernatants from PDC pieces, contaminated and treated or not treated with EDTA and/or taurolidine as detailed above, were tested by HPLC-MS.
b
The indicated pyoverdines were investigated. Using the chromatograms, the different elution peaks were identified, and their areas were used for semiquantitative evaluation of each specific product. The fold change was calculated with respect to the corresponding SB control. The results shown are from a pool of three replicates of a representative experiment out of two performed.
c
ND, not detectable.