FIG. 5.

c-Myc from mitotic HeLa cells does not bind α-tubulin. (A) Anti-c-Myc immunoblot analysis of HeLa cells arrested at mitosis with nocodazole treatment (29) (lane 2) and untreated control (lane 1). One milligram of treated and untreated cells was immunoprecipitated with anti-α-tubulin before immunoblotting with anti-c-Myc (lanes 3 and 4). The arrow between p64 and p67 depicts the hyperphosphorylated c-Myc. IgH, heavy-chain immunoglobulin. (B) The membrane from panel A was stripped (2) and immunoblotted with anti-α-tubulin. (C) In vivo ³²P-labeled HeLa cells arrested at mitosis using a double thymidine block. ³²P-labeled lysates (0.5 mg) were immunoprecipitated (Ipp) with anti-c-Myc or anti-α-tubulin, resolved by SDS-PAGE, and visualized by autoradiography.