Fig. 5. Sub-clustering of genes is responsible for the installation of bioactive chemical features and class-defining chemical features. Gene clusters not shown to scale. (A) Sub-clustering of the DUF and cysteine lyase domains (red) is responsible for the installation of reactive 1,3-dioxo-1,2-dithiolane moiety and is seen in leinamycin (lnm) BGC, guangnanmycin (gnm) BGC, and weishanmycin (wsm) BGC (Section 2.1.4). (B) Sub-clustering of two genes grbD and grbE (blue) that are responsible for the installation of the diazeniumdiolate moiety is evident in the gramibactin BGC (grb) as well as the BGCs responsible for production of megapolibactin from a symbiotic bacterial strain and gladiobactin/plantaribactin from a pathogenic bacterial strain (Section 2.2.1). C. Genes required for installation of the cinnamoyl-moiety (green) are found sub-clustered in the BGCs for WS9326 (cal), skyllamycin (sky), and kitacinnamycin (kcn) (Section 3.3).