TABLE 2.
Metal concentration in wild-type Anabaena and the mutants I-tonB2, I-tonB3, and I-tonB4 expressed as atoms per OD750a
| Metal | 1013 atoms/OD |
Ratio (mutant/WT) |
|||||
|---|---|---|---|---|---|---|---|
| WT | I-tonB2 | I-tonB3 | I-tonB4 | I-tonB2 | I-tonB3 | I-tonB4 | |
| Mg | 500 ± 40 | 410 ± 20 | 350 ± 40 | 280 ± 10 | 0.82 | 0.70 | 0.56 |
| Ca | 61 ± 7 | 70 ± 50 | 60 ± 20 | 40 ± 20 | 1.15 | 0.98 | 0.66 |
| Mn | 51 ± 2 | 17 ± 1 | 11 ± 1 | 30 ± 20 | 0.33 | 0.22 | 0.59 |
| Fe | 34 ± 2 | 35 ± 5 | 29 ± 3 | 30 ± 8 | 1.03 | 0.85 | 0.88 |
| Co | 1.8 ± 0.1 | 1.8 ± 0.1 | 1.2 ± 0.1 | 1.3 ± 0.1 | 1.00 | 0.66 | 0.72 |
| Cu | 5.1 ± 0.5 | 6.7 ± 0.6 | 5 ± 2 | 6 ± 1 | 1.31 | 0.98 | 1.18 |
| Zn | 5.4 ± 0.3 | 6.1 ± 0.4 | 5 ± 4 | 7 ± 3 | 1.13 | 0.93 | 1.29 |
| Mo | 6.4 ± 0.2 | 9.8 ± 0.7 | 7.3 ± 0.6 | 4.8 ± 0.4 | 1.53 | 1.14 | 0.75 |
a
The ratio of the metal content in wild type and mutants is shown. The values represent averages and standard deviation from three biological measurements. The bold letters indicate significant changes in the ratio column (P < 0.05, Student’s t test).