FIG 3.

Resistance to EFdA of HIV-1 mutants selected during passaging. Mutations were introduced into NL4-3. TZM-GFP cells were pretreated with EFdA and infected after 24 h. GFP-positive cells (infected cells) were counted in various concentrations of EFdA. Mutants were normalized to WT infection to produce the fold change. EFdA dose-response curves were produced for each mutant and the EC₅₀s calculated. Statistical significance was determined using one-way ANOVA with Tukey’s posttest (***, P < 0.0001). The results represent the means and SD for four independent experiments performed in duplicate.