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Single-round replication assays using TZM-GFP cells infected with individual mutants. An ELISA was also performed on the virus to determine amount of p24 (total virus in medium). The ratio of infected cells per p24 was then calculated and normalized to the WT. Statistical significance was determined using a one-way ANOVA with Dunnett’s multiple-comparison test (*, P < 0.05; **, P < 0.01). The results represent the means and SD for four independent experiments performed in triplicate.
