TABLE 3.

Methylation identified in the genomes of GAS strains from different emm types

GAS strain	emm type	Motif string	Position modified	Type	% of motifs detected	No. of motifs detected	No. of motifs in genome	Mean modQV
MGAS2221	1	GCANNNNNNTTAA	3	m6A	98.0	337	344	79.09
		TTAANNNNNNTGC	3	m6A	97.7	336	344	77.17
SF370	1	TTAANNNNNNTGC	3	m6A	98.8	331	335	75.26
		GCANNNNNNTTAA	3	m6A	97.9	328	335	76.43
M1-SC	1	ND
MGAS10870	3	CAAYNNNNNNTGC	3	m6A	100.0	524	524	513.45
		GCANNNNNNRTTG	3	m6A	99.8	523	524	493.54
SAS-M4-Duke	4	CAAYNNNNNNTGC	3	m6A	100.0	542	542	143.86
		GCANNNNNNRTTG	3	m6A	100.0	542	542	142.56
RLGH	4	CAAYNNNNNNTGC	3	m6A	100.0	542	542	307.29
		GCANNNNNNRTTG	3	m6A	99.8	541	542	299.71
Duke Large	4	CAAYNNNNNNTGC	3	m6A	100.0	542	542	228.72
		GCANNNNNNRTTG	3	m6A	100.0	542	542	225.93
Duke ΔcovS	4	GCANNNNNNRTTG	3	m6A	98.2	532	542	68.37
		CAAYNNNNNNTGC	3	m6A	96.5	523	542	68.08
TSPY155	11	CRAANNNNNNRTTG	4	m6A	99.8	462	463	283.43
		CAAYNNNNNNTTYG	3	m6A	99.6	461	463	312.70
TSPY136	22	TAAYNNNNNNTCG	3	m6A	100.0	241	241	285.17
		CGANNNNNNRTTA	3	m6A	100.0	241	241	258.73
MGAS6180	28	CRAANNNNNNNTGC	4	m6A	99.5	414	416	203.92
		GCANNNNNNNTTYG	3	m6A	99.0	412	416	229.83
TSPY1309	63	TAAYNNNNNNTGC	3	m6A	100.0	537	537	525.49
		GCANNNNNNRTTA	3	m6A	99.8	536	537	474.90
TSPY416	68	CAAYNNNNNNTGC	3	m6A	100.0	513	513	270.36
		GCANNNNNNRTTG	3	m6A	99.8	512	513	259.95
TSPY453	77	GTAYNNNNNRTTG	3	m6A	100.0	190	190	412.97
		CAAYNNNNNRTAC	3	m6A	100.0	190	190	404.43
TSPY125	83	CAAYNNNNNNCTC	3	m6A	98.9	345	349	476.50
		GAGNNNNNNRTTG	2	m6A	98.0	342	349	458.08
MSPY1	89	TTAANNNNNNTCG	3	m6A	100.0	179	179	312.57
		CGANNNNNNTTAA	3	m6A	99.4	178	179	280.25