TABLE 5.
Primer sequences
| Target | Orientation | Primer sequence (5′–3′) |
|---|---|---|
| Intracellular total HBV DNA | Forward | CCACMWAATGCCCCTATC |
| Reverse | CCCACCTTATGAGTCCAAG | |
| Probe | CRCCGCGTCGCAGAAGATCT | |
| Extracellular HBV total DNA | Forward | CTCGTGGTGGACTTCTCTC |
| Reverse | CACGAYGATGGGATGGGAAT | |
| HBV DNA plus – polarity strand | Forward | CATGAACAAGAGATGATTAGGCAGAG |
| Reverse | GGAGGCTGTAGGCATAAATTGG | |
| Probe | CTGCGCACCAGCACCATGCA | |
| HBV DNA minus – polarity strand | Forward | CTTCTCCGTCTGCCGTT |
| Reverse | GCAGATGAGAAGGCACAGA | |
| Probe | CGCACCTCTCTTTACGCGGACT | |
| HBV cccDNA | Forward | AGGAGGCTGTAGGCATAAATTGGT |
| Reverse | ATTCTTTATAAGGGTCAATGTCCATGC | |
| Probe | ACTGTTCAAGCCTCCAAGCTGTGCCTT | |
| HBV RNA | Forward | CCTCCAAGCTGTGCCTTG |
| Reverse | AARAAGTCAGAAGSCAAAAAWGA | |
| Probe | GGCATGGACATTGACMCBTA | |
| Beta actin | Forward | TTCTACAATGAGCTGCGTGTG |
| Reverse | GGGGTGTTGAAGGTCTCAAA | |
| MT-CO3 | Forward | CCCCACAAACCCCATTACTAAACCCA |
| Reverse | TTTCATCATGCGGAGATGTTGGATGG | |
| pHBV1.1× vector | Forward | CACCAAAATCAACGGGACTT |
| Reverse | AGGCTTGAACAGTGGGACAT |