FIG 4.

YdgH contributes to basal S. marcescens cefoxitin susceptibility. (A) The YdgH locus on the y axis with transposon-insertion (Tn) reads on the y axis, demonstrating the large enrichment of YdgH insertion mutants in cefoxitin (below, compared to the input library; above, on the same scale). (B) The ΔydgH mutant had increased growth in cefoxitin compared to that of the wild type (Wt) at multiple drug concentrations and at multiple time points. (C) Schematic illustrating optical density at 600 nm (OD₆₀₀) ratio used in subsequent figures. The OD₆₀₀ of the ΔydgH mutant at 4 h is divided by that of the Wt. (D) Exogenous ydgH rescues the cefoxitin phenotype in the ΔydgH mutant. *, P ≤ 0.05; **, P ≤ 0.01; unpaired two-tailed t test, unadjusted.