FIG 1.

Characterization of MAbs to chitooligomers. (A) Isotyping of MAbs DD11 and CC5 was performed using culture supernatants of the hybridomas producing each antibody. Results from each hybridoma were compiled; each circle represents the signal obtained from each antibody. The highest signals were obtained for IgM, with MAb DD11 producing the highest reactivity. (B) Denaturing gel of purified MAbs denoting the predominant bands corresponding to the heavy (∼70-kDa) and light (∼23- to 24-kDa) chains of IgM. Lane 1, MAb CC5; lane 2, MAb DD11; lane 3, molecular mass markers.