FIG 4.

Binding of chitooligomer MAbs to whole cells of C. neoformans and C. albicans. The wells of ELISA plates were coated with cells of C. albicans (green lines) or C. neoformans (purple lines) at cell densities ranging from 10² to 10⁷/ml. The reactivities of MAbs DD11 (A) and CC5 (B) at 12.5 μg/ml are shown. Similar tests were performed with MAb DD11 (C) and MAb CC5 (D) with G. lamblia (green lines), A549 human cells (orange lines), Gram-negative E. coli (purple lines), and Gram-positive S. aureus (gray lines). Dot blotting also shows the binding of chitooligomer MAbs to whole cells of C. neoformans and C. albicans. MAbs DD11 (E) and CC5 (F) were tested against whole cells of C. albicans (green lines) and C. neoformans (purple lines) at various cell densities. The MAbs were used at 12.5 μg/ml. The results illustrate a representative experiment, with three independent replicates producing similar results.