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Treatment of C. neoformans with chitooligomer MAbs results in increased intracellular staining of the fungus with propidium iodide. C. neoformans was treated with MAb DD11 (A, purple histogram) or CC5 (B, red histogram) at 12.5 μg/ml for 2 h at 37°C, stained with 0.5 μg/ml propidium iodide, and immediately analyzed by flow cytometry. Control systems (no antibody; orange histograms) were incubated in PBS. These results represent two independent replicates that produced similar results.
