FIG 5.
Effects of stilbenoids on infectious SARS-CoV-2 replication in vitro using a CPE scoring-based assay. (A) Representative images of Vero-E6 cells infected with 50× TCID50 of SARS-CoV-2 (USA-WA1/2020 variant) at 0 to 4 days postinfection. Arrows denote examples of CPE. (B) Representative images of infected cells in the presence of REGN10933 at the stated concentrations. (C) Dose-response curve of REGN10933 on viral replication in Vero-E6 cells after 4 days of infection. (D) Dose-response curve of REGN10933 on cell viability in uninfected Vero-E6 cells after 4 days of incubation. (E) Representative images of infected cells in the presence of remdesivir (top) and hopeaphenol (bottom) after 4 days of incubation at the stated concentrations. (F) Dose-response curves of stilbenoids, resveratrol, and remdesivir and GC-376 controls on viral replication in Vero-E6 cells after 4 days of infection. (G) Dose-response curves of compounds on cell viability in uninfected Vero-E6 after 4 days of incubation. In panels A, B, and E, scale bars = 100 μm. Results in panels C, D, F, and G denote the mean ± SEM from at least 3 independent experiments.