FIG. 5.

SSA in a rad59 msh3 double mutant. (A) HO endonuclease initiates SSA by cleaving between 415-bp sequences (black boxes) of ura3, resulting in a deletion product. (B) Wild-type (WT; tNS759), msh3 (tNS1551), rad59 (tNS1789), and msh3 rad59 (tNS1967) strains were induced for 5 h with galactose. At 0 and 5 h, DNA samples were obtained, digested with BglII, and probed with a sequence adjacent to ura3 (cross-hatched box in panel A), revealing the uncut band (8.6 kb), the HO-cut fragment (4.8 kb), and the product band (5.5 kb).