TABLE 1.
Strains used in this study
| Strain | Genotypea |
|---|---|
| JKM146 | Δho Δhml::ADE1 MATa-inc Δhmr::ADE1 ade1 leu2-3,112 lys5 trp1::hisG ura3-52 ade3::GAL::HO |
| tNR85 | Δho HML matΔ::LEU2 hmrΔ3 leu2-3,112 trp1 thr4 ura3-52 pFH800 |
| tNS709 | tNR85 (THR4 ura3 [29 bp]-cut site-URA3) |
| tNS747 | tNR85 (THR4 ura3 [142 bp]-cut site-URA3) |
| tNS759 | tNR85 (THR4 ura3 [415 bp]-cut site-URA3) |
| tNS1103 | tNR85 (THR4 ura3 [205 bp]-cut site-URA3) |
| tNS1110 | tNR85 (THR4 ura3 [235 bp]-cut site-URA3) |
| tNS1142 | tNR85 (THR4 ura3 [63 bp]-cut site-URA3) |
| tNS1157 | tNR85 (THR4 ura3 [89 bp]-cut site-URA3) |
| tNS1379 | Δho HML matΔ::leu2::hisG hmrΔ3 leu2-3,112 ura3-52 trpl thr4 (ura3 [205 bp]-cut site-URA3) pFH800 |
| tNS1530 | tNR85 (THR4 ura3 [1,170 bp]-cut site-URA3) |
| tNS1551 | tNS759 msh3::KanMX |
| tNS1573 | tNS1379 rad59::KanMX |
| tNS1585 | tNS1379 srs2::KanMX2 |
| tNS1597 | tNS1379 rad51::LEU2 |
| tNS1703 | JKM146 rad59::KanMX2 |
| tNS1706 | JKM146 msh3::LEU2 rad59::KanMX2 |
| tNS1712 | tNS1530 rad59::KanMX2 |
| tNS1785 | tNS759 rad59::KanMX2 |
| tNS1853 | JKM146 srs2::LEU2 rad59::KanMX2 |
| tNS1967 | tNS759 rad59::NatMX4 msh3::KanMX |
| tNS1984 | tNS709 rad59::KanMX2 |
| tNS1990 | tNS709 rad52::KanMX2 |
| YFP218 (tNS1924) | JKM146 srs2::LEU2 |
| YFP259 | JKM146 msh3::LEU2 |
a
Cut site refers to the 117-bp BglII-HincII MATa HO endonuclease recognition site.