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. Author manuscript; available in PMC: 2021 Nov 17.
Published in final edited form as: J Carbohydr Chem. 2012 Jan 19;31(1):10–30. doi: 10.1080/07328303.2011.626544

Table 3:

Comparative profiles of permethylated O-linked glycans of five different CA215 samples

Sample ID Observed mass m/z [M+Na]+ Proposed structure Structure
CA215 (lots: A, B, and C)a 534 GalNAc1Gal1 graphic file with name nihms-816278-t0001.jpg C
CA215 (lots: A and B) 708 GalNAc1Gal1Fuc1 graphic file with name nihms-816278-t0002.jpg
CA215 (lots: A, B, D, C, E, and F)b 896 GalNAc1Gal1NeuAc1 graphic file with name nihms-816278-t0003.jpg
CA215 (lots: C, E, and F) 926 GalNAc1Gal1 NeuGc1 graphic file with name nihms-816278-t0004.jpg
CA215C 940d GalNAc1GlcNAc1NeuAc1 graphic file with name nihms-816278-t0005.jpg
CA215 (lots: A, B, and C) 1140 GalNAc1GlcNAc1Gal1NeuAc1 graphic file with name nihms-816278-t0006.jpg
CA215 (lots: C, D, E, and F) 1257 GalNAc1Gal1NeuAc2 graphic file with name nihms-816278-t0007.jpg
CA215 (lots: C, E, and F) 1317 GalNAc1Gal1NeuGc2 graphic file with name nihms-816278-t0008.jpg
CA215 (lots: A, B, C, E, and F) 1345 GalNAc1GlcNAc1Gal2NeuAc1 graphic file with name nihms-816278-t0009.jpg
CA215 (lots: C, E, and F) 1375 GalNAc1GlcNAc1Gal2NeuGc1 graphic file with name nihms-816278-t0010.jpg
a

CA215 lots A, B, and C were from OC-3-VGH ovarian cancer cells (CA215-OC-3); lots A and B were obtained through acid elution, whereas lots C, D, E, and F were obtained through elution with 3 M urea.

b

Lot CA215D was obtained by an additional purification of urea-eluted CA215 (S15K-100425) with goat antihuman IgG affinity column followed by the same analysis. CA215 lots E and F were from C-33A cervical cancer cells (CA215-C33A).

c

N-acetylgalactosamine (□), N-acetylglucosamine (■), Fucose (▲), Galactose (Inline graphic), N-acetylneuraminic acid (Inline graphic), and N-glycolylneuraminic acid (Inline graphic).

d

Detected by MALDI-TOF MS method but not found by NSI-MS method.