Fig. 2. (A) Schematic illustration of the oligomerisation process of Hsp70. Components of Hsp70 are shown as follows: nucleotide binding domain (blue), substrate binding subdomain (red), helical lid subdomain (grey), and interdomain linker (green). Under physiological conditions in vitro, Hsp70 exists in equilibrium between monomeric, dimeric, and higher order oligomeric species.52 Reproduced from ref. 52. (B) (i) Oligomeric population distribution of αBC obtained by quantitative mass spectrometry measurements.71 Reproduced from ref. 71. (ii) Modes of interaction between αBC molecules. Intra-dimer interactions hold two αBC molecules together, and inter-dimer interactions link adjacent dimeric subunits. The result is an overall polyhedral αBC architecture. Reproduced from ref. 68.
